|
Tissue Source
|
Liver
|
Disease/Type
|
Hepatocellular Carcinoma
|
|
Morphology
|
Epithelial-like
|
Growth Properties
|
Adherent
|
|
Culture Medium
|
EMEM
|
Serum
|
10% FBS
|
|
Subculture Ratio
|
1:4~1:6
|
Passage Frequency
|
2 Times/Week
|
|
Doubling Time (h)
|
~48
|
Cryopreservation
|
Medium + 5% DMSO
|
|
Repository
|
ATCC/DSMZ/JCRB
|