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A randomized phase 3 trial of Gemcitabine or Nab-paclitaxel combined with cisPlatin as first-line tr

Issuing time:2022-07-15 09:37

Abstract

Platinum is recommended in combination with gemcitabine in the treatment of metastatic triple-negative breast cancer (mTNBC). We conduct a randomized phase 3, controlled, open-label trial to compare nab-paclitaxel/cisplatin (AP) with gemcitabine/cisplatin (GP) in mTNBC patients (ClinicalTrials.gov NCT02546934). 254 patients with untreated mTNBC randomly receive AP (nab-paclitaxel 125 mg/m² on day 1, 8 and cisplatin 75 mg/m² on day 1) or GP (gemcitabine 1250 mg/m² on day 1, 8 and cisplatin 75 mg/m² on day 1) intravenously every 3 weeks until progression disease, intolerable toxicity or withdrawal of consent. The primary endpoint is progression-free survival (PFS); secondary endpoints are objective response rate (ORR), safety and overall survival (OS). The trial has met pre-specified endpoints. The median PFS is 9.8 months with AP as compared to 7.4 months with GP (stratified HR, 0.67; 95% CI, 0.50–0.88; P = 0.004). AP significantly increases ORR (81.1% vs. 56.3%, P < 0.001) and prolongs OS (stratified HR, 0.62; 95% CI, 0.44–0.90; P = 0.010) to GP. Of grade 3 or 4 adverse events, a significantly higher incidence of neuropathy in AP and thrombocytopenia in GP is noted. These findings warrant further assessment of adding novel agents to the nab-paclitaxel/platinum backbone due to its high potency for patients with mTNBC.

Introduction

Triple-negative breast cancer (TNBC), lacks the expression of estrogen receptor (ER) and progesterone-receptor (PgR) and amplification of human epidermal growth factor receptor 2 (HER2) gene, accounts for up to 20% of all breast cancers, and is associated with aggressive behavior and poor prognosis1. Patients with TNBC are more likely to experience earlier relapse and shorter survival time as compared to those with other subtypes2. While endocrine therapy and HER2-targeted agents have significantly increased the survival benefit of luminal and HER2-positive metastatic breast cancer, the prognosis of TNBC remains poor. Although a variety of novel therapeutic strategies emerged in recent years, the median survival time for metastatic TNBC is still about 12–25.7 months3,4,5,6,7.

TNBC shares molecular characteristics with basal-like breast cancer8,9. About 10–15% TNBCs carried BRCA mutation10,11, while 47.7–71.0% harbored a deficiency in homologous recombination6,12. These phenomena provide a foundation for DNA cross-linking agents, such as platinum in the treatment of TNBC. The platinum treatment has aroused interest in early and advanced TNBCs, and evidence suggests that platinum-containing regimens are clinically beneficial to patients with TNBC. In the preoperative setting, the addition of platinum to neoadjuvant chemotherapy increased pathological complete response (pCR)13,14, while prolonged progression-free survival (PFS) has also been observed among metastatic TNBC (mTNBC) patients treated with platinum-containing regimens4,15,16. Currently, platinum agents, carboplatin and cisplatin, have been recommended in combination with gemcitabine in the treatment of mTNBC17,18,19.

Our previous study has proven the superiority of gemcitabine/cisplatin (GP) in first-line treatment of mTNBC in terms of PFS, compared with gemcitabine/paclitaxel (7.7 months for GP and 6.5 months for GT group, but no significant difference in OS)15. Thus, GP has been recommended in the guidelines of Chinese Breast Cancer Society and German Gynecological Oncology Working Group18,19. To further exploit the potential effect of cisplatin for TNBC, we chose nanoparticle albumin-bound (nab)-paclitaxel as the partner since it exerts the highest anti-tumor activity for metastatic breast cancer (MBC)20. Furthermore, the safety profile and activity of nab-paclitaxel/cisplatin (AP) were tested in patients with mTNBC in our previous prospective phase 2 study21. Based on the evidence of high response of nab-paclitaxel and synergistic effect of nab-paclitaxel and cisplatin, we hypothesized a superior efficacy of AP to GP as first-line treatment for patients with mTNBC, and the trial protocol was approved by Celgene Global Medical in 2015.

In this work, we report the results of GAP, a multicenter, randomized, open-label, first-line phase 3 trial to compare the efficacy of first-line AP to GP in mTNBC patients. We show an improved PFS with AP compared to GP as first-line treatment in patients with mTNBC.

Results

Patients

A total of 254 patients were enrolled and randomly assigned to receive AP (n = 127) or GP (n = 127) between 30 March 2016 and 09 October 2019 (Fig. 1). One patient in the GP group did not receive the assigned treatment because of consent withdrawal. Thus, 253 patients, who received at least one dose of the study drugs (127 assigned to AP and 126 to GP), were included in the ITT and safety analysis. Patient demographics and baseline disease characteristics were well-balanced between the treatment groups (Table 1).

Fig. 1: Consort diagram of the GAP study.
figure 1

Patients may have discontinued the assigned treatment for multiple reasons. This figure shows the numbers of patients included in the intention-to-treatment and safety analyses.

Table 1 Patient characteristics.

Treatment exposure

Patients received a median of six cycles of treatment in both AP and GP groups (range: 1–10 for the AP group and 0–10 for the GP group). 241 (95.2%) of 253 patients underwent at least two cycles of treatment in this study. The relative dose intensity was 92 and 95% for nab-paclitaxel and cisplatin, respectively, and 92 and 94% for gemcitabine and cisplatin, respectively (Supplementary Table 1). At the cutoff point of the analysis (23 Feb 2021), the treatment has discontinued in both groups (Fig. 1). 26 (20.4 %) of 127 patients in the AP group and 47 (37.3%) of 126 patients in the GP group discontinued the treatment due to disease progression. Dose reduction occurred in 21 (16.5%) of 127 patients in the AP group and 47 (37.3%) of 126 patients in the GP group.

Efficacy

At the cutoff date of data analysis, a total of 202 patients, 101 in the AP group and 101 in the GP group, experienced disease progression or death. The median follow-up was 23.2 months for the AP group and 19.3 months for GP group in the whole population. PFS was significantly longer in the AP group than in the GP group (median, 9.8 months [95% CI 8.70–10.90] vs. 7.4 months [95% CI 5.93–8.94]; HR, 0.69; 95% CI 0.53–0.92; P = 0.010; stratified HR, 0.67; 95% CI 0.50–0.88; P = 0.004) (Fig. 2A).

Fig. 2: Kaplan–Meier estimates of survival curves for AP and GP groups.
figure 2

Kaplan–Meier plot of PFS (A) and OS (B). Data for the ITT population. Treatment effects were compared using the log-rank test; HRs and corresponding 95% CIs were estimated using Cox-proportional-hazard regression model. P values are one-sided with no adjustment for multiplicity. HR = hazard ratio.

For subsequent medical treatments after disease progression (Supplementary Table 5), 64 (63.4%) of 101 patients in the AP group and 54 (53.4%) of 101 patients in the GP group received subsequent treatments. Among them, 24 (37.5%) and 29 (53.7%) patients received vinorelbine-containing combination as second-line therapy in the AP and GP group, respectively. Moreover, 11 (9.5%) patients received bevacizumab, and 10 (18.5%) patients received a taxane-based treatment in the GP group.

Other key subgroups and exploratory analyses of PFS by stratification factors are shown in Fig. 3. The median PFS was significantly longer with the AP group than with the GP group in the majority of subgroups. In the ITT population, ORR, as assessed by the investigator, was 81.1% (103/127) in the AP group as compared to 56.3% (71/126) in the GP group (P < 0.001) (Table 2), while ORR by central assessment was 79.5% (101/127) and 58.7%, respectively (P < 0.001) (Supplementary Table 2). The median duration of response was 8.4 months (95% CI, 7.1–9.7) in patients treated with AP and 6.4 months (95% CI, 4.4–8.4) with GP (HR, 0.68; 95% CI 0.48– 0.95). At the time point of the data cutoff, 55 (44.3%) of 127 patients in the AP group and 67 (53.2%) of 126 in the GP group had died. OS was significantly different between two groups with the stratified HR of 0.62 (95% CI 0.44–0.90; P = 0.01; HR, 0.67; 95% CI 0.47–0.96; P = 0.028) and median OS was 26.3 months for the AP group and 22.9 months for the GP group (Fig. 2B).

Fig. 3: Subgroup analysis of PFS.
figure 3

Data are median for ITT population in clinically relevant subgroups. Subgroup included patients with <40 years (n = 45) and ≥40 years (n = 208); ECOG = 0 (n = 40) and ECOG = 1 (n = 213); premenopausal status (n = 113) and postmenopausal status (n = 140); de novo stage IV (n = 48), disease-free survival <1 year (n = 29) and ≥1 year (n = 176); No. of metastatic organ site = 1 (n = 94), =2 (n = 81) and ≥3 (n = 78); visceral metastasis (n = 163) and no visceral metastasis (n = 90); previous treatment with anthracycline (n = 173) and with no anthracycline (n = 80); previous treatment with taxane (n = 170) and with no taxane (n = 83). HRs and corresponding 95% CIs were estimated using Cox-proportional-hazard regression model. Data are presented as HR and 95% CI. HR hazard ratio, ECOG Eastern Cooperative Oncology Group.

Table 2 Locally assessed response to study treatment in ITT population.

Safety

All 253 patients in the safety population developed at least one AE (Table 3). All-grade AE that was at least 5% greater in the AP arm were neutropenia, neuropathy, anorexia, fatigue, nausea, and vomiting, and hypomagnesemia, however for grade ≥3 only neuropathy (19% vs 0%) and nausea and vomiting (6% vs 1%) appeared to markedly differ. Conversely, the GP arm had at least 5% more grade ≥3 thrombocytopenia (29.4% vs. 3.9%). Neutropenia, neuropathy, and anemia were the most commonly reported grade 3 or 4 AEs in the AP group, with a frequency of 40.9%, 18.9%, and 15.0%, respectively. The most commonly reported grade 3 or 4 AEs with GP were neutropenia (40.5%), thrombocytopenia (29.4%), and anemia (11.9%). SAEs were reported in 6 (4.7%) of 127 patients in the AP group and for 4 (3.2%) of 126 patients in the GP group. No treatment-related deaths were reported, while 2 patients died during the treatment of AP; one was due to the concurrent uncontrolled type 2 diabetes mellitus with sudden ketoacidosis, and the other was due to meningeal metastasis.

Table 3 AEs.

Discussion

As the first-line treatment, AP significantly improved PFS, as compared to GP in the ITT population with a reduction in the risk of progression or death by 34%. Importantly, we observed a 4.3 months increase in survival in AP group. AP also led to significantly higher ORR than that for gemcitabine and cisplatin. As expected, both combinations of the drugs were consistent with the known toxic effects of each agent.

To the best of our knowledge, AP in this trial reached the numerically longest PFS and highest response rate among the phase 3 studies in the first-line treatment of mTNBC, with the median PFS of 9.8 months and the ORR of 81.1%. Immune checkpoint inhibitors, such as atezolizumab, a PD-L1 antibody3,22, and pembrolizumab, a PD-1 antibody23, achieved clinically significant PFS and/or OS benefits (Supplementary Table 3). However, both were established on the basis of the relatively weaker control regimen with a median PFS of only 5.0–5.6 months3,23, whereas GP doublet in this trial had a median PFS of 7.4 months. In addition, a quick response was realized with the progressive disease of only 0% (0/127), 2.4% (3/127), and 9.4% (12/127) at the end of cycles 2, 4, and 6, respectively (Supplementary Table 4). TNBC is an aggressive disease and usually requires a highly potent regimen to reduce the tumor burden and relieve the symptoms swiftly, which might in part be the reason for the success of the AP doublet. Lastly, the AP doublet was covered in the healthcare systems of China and may provide an affordable option for patients who are in poor economic conditions.

This study further confirmed the valuethe of first-line AP doublet. The efficacy of GP was similar in this trial to our previous phase 3 trial (7.4 and 7.73 months, respectively). This not only reaffirmed the anti-tumor activity of the GP regimen for mTNBC but based on the results from the two studies, a favorable efficacy of AP could be ascribed to paclitaxel plus gemcitabine by indirect comparison, further supporting the efficacy of AP regimen for patients with mTNBC. Nab-paclitaxel combined with carboplatin in the tnAcity trial, a randomized phase 2 trial, also confirmed the application of nab-paclitaxel and platinum for mTNBC5. Typically, for the biologically and molecularly similar cancer types with TNBC, such as serous ovarian cancer and lung squamous carcinoma24, the doublet of taxane and platinum is the first-line standard of care. In TNBC, the addition of bevacizumab to nab-paclitaxel/carboplatin in a phase 2 trial reached a PFS of 9.2 months and an ORR of 85%25, whereas adding pembrolizumab to paclitaxel/carboplatin followed by anthracycline plus cyclophosphamide in a phase 3 trial increased the pCR rate by 13.6%26. Therefore, it is reasonable to hypothesize that the first-line nab-paclitaxel/platinum doublet, rather than single-agent chemotherapy, may be the appropriate chemotherapy backbone to explore the addition of novel agents.

AP doublet translated its improvements in efficacy into a significant survival benefit. The reduction in risk of death may result from the higher anti-tumor activity of nab-paclitaxel over gemcitabine when combined with cisplatin. We first identified the survival benefit of chemotherapy doublet to another doublet in mTNBC population. Nevertheless, we don’t know if AP may achieve the improved OS to nab-paclitaxel crossed over to cisplatin at the same doses and schedule.

Whether combination treatment or sequential treatment is optimal for mTNBC patients has been causing of discussion. Previous studies demonstrate that combination therapy brings no significant survival benefit and increased toxicity compared with sequential single-agent therapy, despite improving response rates and PFS27. However, the population of these studies included all subtypes, not limited to mTNBC. It is uncertain if mTNBC may benefit from a different approach due to its aggressive behavior with a higher tendency for heavy tumor burden and visceral metastasis. Furthermore, for first-line treatment of mTNBC, combination therapy is regularly considered both in clinical practice and clinical trials5,23,28. For example, in KEYNOTE-355 study23, 55% of investigators chose gemcitabine plus carboplatin as the treatment of physician choice. For these reasons, we explored first-line combination chemotherapy for patients with mTNBC in our serial studies (NCT02341911)15. In this study, we provided more evidence for combination therapy and identified cisplatin as an alternative partner for combination therapy.

As expected, neuropathy rates were higher in the AP group than in the GP group (63% any-grade and 19% grade 3 for AP group), which could be attributed to nab-paclitaxel combined with concomitant neurotoxic cisplatin and the long exposure to both drugs. In our previous phase 2 trial, nab-paclitaxel (125 mg/m2 on d1, d8 and d15) plus cisplatin (75 mg/m2 on d1) every 28 days led to a peripheral neuropathy of 72.6% for any-grade and 26.0% for grade 3. Decreased relative dose intensity of nab-paclitaxel in this study seemed to reduce the incidence of peripheral neuropathy. Whereas, the tnAcity trial reported that grade 3 peripheral neuropathy of nab-paclitaxel plus carboplatin was 5% in 64 patients (the same dose of nab-paclitaxel with this study), indicating that cisplatin-induced peripheral neurotoxicity is more frequent and severe in conventional doses. Neuropathy should be considered when making decisions on the first-line treatment regimen. In general, most toxic effects of the AP were minor, rarely limited therapy. More patients experienced dose reduction in GP group than AP group (37.3% vs.16.5%) and patients who had treatment discontinuation were similar in the two groups (19.0% vs. 22.8%).

The present study also has some limitations. The sample size was evaluated based on the primary endpoint of PFS with 80% power, which might be less powered for a phase 3 trial, and the sample size might not provide a full toxicity profile of AP regimen. A larger population is warranted to further identify the PFS and OS benefits of AP in mTNBC patients. Secondly, a retrospective translational study is being carried out, but we didn’t obtain all samples from patients because the translational part had not been prospectively designed.

In conclusion, the AP doublet in patients with mTNBC, compared to GP, achieved greater efficacy with manageable toxicity, and hence, is an option as a first-line treatment. In the context of all the available first-line evidence, this doublet could also serve as the chemotherapy backbone to further assess novel agents due to the high potency for all-comers of mTNBC. A study of AP combined with PD-1 antibody has been initiated in our center to further improve the efficacy and survival benefit of this regimen.


Article classification: Biological abstract
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