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ELISA kit experimental operation skills

Issuing time:2019-08-08 16:20Author:XinqidibioSource:www.enlibio.com

ELISA kit experimental operation skills


There are many kinds of ELISA kits in China, such as ELISA test kit, ELISA kit, enzyme-linked immunosorbent kit, enzyme-linked immunosorbent assay kit, enzyme-linked immunosorbent assay kit, enzyme-free kit, etc. It is called ELISA test kit, enzyme-linked immunosorbent assay kit, etc.; ELISA kit is suitable for qualitative detection of target proteins or other analytes in serum or plasma samples, since the advent of the 60s and 70s, The recognition and admiration of scientific researchers all over the world has been greatly promoted in Europe, America and China, especially in the domestic biochemical field. The Elisa biological test is a highly sensitive, specific, and reproducible experimental diagnostic method. Because of its stable reagents, easy storage, easy operation, and objective judgment, it has been widely used in various fields of immunology. In order to ensure the quality of the ELISA kit experiments, we need to master some tips to help the success of the experiment, then you know the operation skills of the ELISA kit?


Wuhan Xinqidi Biotech Co., Ltd. summarized 17 kinds of operation skills of ELISA kit. If you master these experimental techniques, you will be more proficient in the ELISA experiment, which greatly reduces the risk during the experiment. ELISA kit After getting started with the knowledge of the experimental beginners, the operation will be much faster.


The 17 related operation skills of the ELISA kit are as follows:


1. Remember to use a blotting paper to gently blot and dry on the surface of the microplate after adding the enzyme reagent.

2. Arrange the amount of detection reasonably so as to avoid long waiting time for washing the plate.

3. When sucking the liquid, it is necessary to remove the gun with a dose and a quantity close to the required amount to reduce the error.

4. Be sure to do a two-hole experiment so that the accuracy of the data can be guaranteed and the precision of the kit can be reflected.

5. The sample diluent is filled with a pipette and often corrected for accuracy.

6. In order to prevent the sample from evaporating, the reaction plate is placed in a closed box covered with a damp cloth, and the plate is covered with a cover or a film.

7. Unused plate or reagent, please store at 2-8 °C. Please use the horseradish peroxidase working solution according to the required amount. Do not reuse the diluted horseradish peroxidase working solution.

8. In the ELISA kit experiment, the incubator should be placed in time after the sample is added. When there are more specimens, it must be operated in batches. According to the instructions, the operation time is strictly controlled to prevent the incubation time from being artificially prolonged, resulting in non-specific binding surrounding the reaction well, which is difficult to clean thoroughly.

9. The remaining samples and waste should be sterilized by autoclaving at 121 °C for 30 minutes, or treated with a disinfectant such as 5.0 g/L sodium hypochlorite for 30 minutes.

10. When the ELISA kit is washed, each well needs to be filled with liquid to prevent the free enzyme in the orifice from being washed.

11. One hole was left for each experiment as a blanking zero hole. No additional reagent was added to the well, except that the substrate solution and 2N H2SO4 were added. Use this hole to adjust the OD value to zero when measuring.

12. After washing the plate manually, add the washing solution each time. Should be allowed to stand for 15 ~ 30s, do not splash the washing liquid in one of the enzyme standard pores into another enzyme labeled hole to prevent cross-contamination. After removing the washing solution, place the microplate on a towel or absorbent paper and pat dry.

13. When there is doubt about the results of the sample, other test methods need to be used for verification.

14. Prepare the solution with deionized water or double distilled water. Do not use tap water.

15. When using the micro-sampler, replace the tip with the liquid in different bottles, even if the standard solution is taken.

16. When the liquid is sucked, the speed is not too fast, so as to avoid air bubbles, and the amount of the ELISA kit is not accurate enough.

17. When sucking the liquid, use a micro-sampler with a close range and a required amount to reduce the error.


For more technical questions, please contact Wuhan Xinqidi Biotechnology Co., Ltd.

Contact: Room A11-329, 1st Floor, No.1, SBI Venture Street, Guanggu, Donghu New Technology Development Zone, Wuhan

Tel: 027-87610298, 027-87610297

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